Identification and interrogation of combinatorial histone modifications
نویسندگان
چکیده
منابع مشابه
Identification and interrogation of combinatorial histone modifications
Histone proteins are dynamically modified to mediate a variety of cellular processes including gene transcription, DNA damage repair, and apoptosis. Regulation of these processes occurs through the recruitment of non-histone proteins to chromatin by specific combinations of histone post-translational modifications (PTMs). Mass spectrometry has emerged as an essential tool to discover and quanti...
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P osttranslational modifications of histones have attracted enduring interest ever since it was realized that histones are hyperacetylated on lysines at actively transcribed genes (1). Because it neutralizes the charge on a lysine, acetylation was thought to reduce interactions with DNA phosphates, making the DNA more accessible for active processes such as transcription (2). In recent years, t...
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Nucleosomes are involved in DNA compaction and transcriptional regulation. Yet it is unclear whether histone modification marks are primary or secondary to transcription and whether they interact to form a histone code. We investigated the relationship between transcription and four histone modifications (H4ac, H3ac, H3K4me2/3) using ChIP-chip and expression microarray readouts from two murine ...
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Histone post-translational modifications (PTMs) often form complex patterns of combinations and cooperate to specify downstream biological processes. In order to systemically analyse combinatorial PTMs and crosstalks among histone PTMs, we have developed a novel nucleosome purification method called Biotinylation-assisted Isolation of CO-modified Nucleosomes (BICON). This technique is based on ...
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ژورنال
عنوان ژورنال: Frontiers in Genetics
سال: 2013
ISSN: 1664-8021
DOI: 10.3389/fgene.2013.00264